cfx96 touchtm realtime pcr detection system (Bio-Rad)
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cfx96 touchtm realtime pcr detection system
Cfx96 Touchtm Realtime Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx96+touchtm+realtime+pcr+detection+system/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm41831658-58-44-50
Average 96 stars, based on 27901 article reviews
Cfx96 Touchtm Realtime Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx96+touchtm+realtime+pcr+detection+system/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm41831658-58-44-50
Average 96 stars, based on 27901 article reviews
cfx96 touchtm realtime pcr detection system - by Bioz Stars,
2026-09
96/100 stars
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Purification:Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals. Article Snippet: Quantitative PCR For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (BioRad) using 1 g of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals Article Snippet: For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (Bio-Rad) using 1 μg of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a Reverse Transcription:Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals. Article Snippet: Quantitative PCR For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (BioRad) using 1 g of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a Article Title: Human serum albumin and biological fluids reduce cefiderocol activity in carbapenem-resistant Gram-negative bacteria. Article Snippet: RNA extraction was performed from three independent biological replicates using the Direct-zol RNA Kit (ZymoResearch), according to the manufacturer’s instructions. .. DNase-treated RNA was used for complementary DNA (cDNA) synthesis with the iScriptTM Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). cDNA was adjusted to 50 ng/μL and used as template for qRT-PCR with qPCRBIO SyGreen Blue Mix Lo-ROX (PCR Biosystems, Wayne, PA, USA) on a Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals Article Snippet: For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (Bio-Rad) using 1 μg of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a cDNA Synthesis:Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals. Article Snippet: Quantitative PCR For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (BioRad) using 1 g of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a Article Title: Human serum albumin and biological fluids reduce cefiderocol activity in carbapenem-resistant Gram-negative bacteria. Article Snippet: RNA extraction was performed from three independent biological replicates using the Direct-zol RNA Kit (ZymoResearch), according to the manufacturer’s instructions. .. DNase-treated RNA was used for complementary DNA (cDNA) synthesis with the iScriptTM Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). cDNA was adjusted to 50 ng/μL and used as template for qRT-PCR with qPCRBIO SyGreen Blue Mix Lo-ROX (PCR Biosystems, Wayne, PA, USA) on a Article Title: Co-treatments of vitamin D and mesenchymal stem cells effectively alleviate the diabetic kidney disease through attenuating the SIRT1-mediated pathways. Article Snippet: Total RNA was extracted from the renal tissues and MPC5 related cells using the Trizol reagent (TaKaRa, Japan). .. Their cDNA samples were then reversely transcribed by the Transcript® One-Step gDNA Removal and cDNA Synthesis SuperMix (no. AT341-02, TransGen Biotech, China), and performed by the qRT-PCR method using the Top Green qPCR SuperMix (AQ131-01, TransGen Biotech, China) under the Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals Article Snippet: For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (Bio-Rad) using 1 μg of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a Real-time Polymerase Chain Reaction:Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals. Article Snippet: Quantitative PCR For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (BioRad) using 1 g of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a Article Title: Sequential YAP1/FOSL1 silencing and epigenetic therapy to overcome stromal barriers in pancreatic cancer. Article Snippet: Total RNA was extracted from MIA PaCa-2, mCAF and hCAF cells using PureLinkTM RNA Mini Kit (Invitrogen) according to the manufacturer’s instructions. cDNA synthesis was performed using the SuperScriptTM III Reverse Transcriptase (Invitrogen) with 1 μg of total RNA as the template. .. Real-time PCR was carried out using SYBRTM Green PowerUpTM Master Mix (Applied Biosystem) on a Article Title: Targeted destruction of follicle stimulating hormone receptor-positive cancer cells in vitro and in vivo by a lytic peptide Phor21-FSHβ conjugate. Article Snippet: One microgram of RNA was treated with DNase I (Sigma‒Aldrich) and reverse-transcribed using the SensiFAST cDNA Synthesis Kit (Bioline, London, UK) at 48 °C for 60 min (for highly structured RNA). .. Real-time quantitative polymerase chain reaction (qPCR) was performed using the DyNAmo HS SYBR Green qPCR Kit (Thermo Fisher Scientific) with the following primers: PPIA (144 bp), F: G C C A A G A C T G A G T G G T T G G A T G; R: G A G T T G T C C A C A G T C A G C A A T G G; FSHR exon 1–2 (145 bp); F: T G G G C T C A G G A T G T C A T C A T C G G A; R: T G G A T G A C T C G A A G C T T G G T G A G G; FSHR exon 5–7 (136 bp); F: A A G C A C C T T C C A G A T G T T C A C; R: T C A G C C A T A G A A T C A C A C T T T C A; GNRHR (gonadotropin releasing hormone receptor) (116 bp), F: C A G A G C C C T T T G C C C A T A A T A; R: T G G T T A C T G A C T C C T C C A A A T G; GNRHR (103 bp), F: T G G C A C A A C T A A C A C C T A C T G; R: G A G G T A G A C A C A A G C C T G A A T T A. qPCR was performed using the Article Title: Co-treatments of vitamin D and mesenchymal stem cells effectively alleviate the diabetic kidney disease through attenuating the SIRT1-mediated pathways. Article Snippet: Total RNA was extracted from the renal tissues and MPC5 related cells using the Trizol reagent (TaKaRa, Japan). .. Their cDNA samples were then reversely transcribed by the Transcript® One-Step gDNA Removal and cDNA Synthesis SuperMix (no. AT341-02, TransGen Biotech, China), and performed by the qRT-PCR method using the Top Green qPCR SuperMix (AQ131-01, TransGen Biotech, China) under the Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals Article Snippet: For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (Bio-Rad) using 1 μg of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a Polymerase Chain Reaction:Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals. Article Snippet: Quantitative PCR For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (BioRad) using 1 g of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a Article Title: Sequential YAP1/FOSL1 silencing and epigenetic therapy to overcome stromal barriers in pancreatic cancer. Article Snippet: Total RNA was extracted from MIA PaCa-2, mCAF and hCAF cells using PureLinkTM RNA Mini Kit (Invitrogen) according to the manufacturer’s instructions. cDNA synthesis was performed using the SuperScriptTM III Reverse Transcriptase (Invitrogen) with 1 μg of total RNA as the template. .. Real-time PCR was carried out using SYBRTM Green PowerUpTM Master Mix (Applied Biosystem) on a Article Title: Targeted destruction of follicle stimulating hormone receptor-positive cancer cells in vitro and in vivo by a lytic peptide Phor21-FSHβ conjugate. Article Snippet: One microgram of RNA was treated with DNase I (Sigma‒Aldrich) and reverse-transcribed using the SensiFAST cDNA Synthesis Kit (Bioline, London, UK) at 48 °C for 60 min (for highly structured RNA). .. Real-time quantitative polymerase chain reaction (qPCR) was performed using the DyNAmo HS SYBR Green qPCR Kit (Thermo Fisher Scientific) with the following primers: PPIA (144 bp), F: G C C A A G A C T G A G T G G T T G G A T G; R: G A G T T G T C C A C A G T C A G C A A T G G; FSHR exon 1–2 (145 bp); F: T G G G C T C A G G A T G T C A T C A T C G G A; R: T G G A T G A C T C G A A G C T T G G T G A G G; FSHR exon 5–7 (136 bp); F: A A G C A C C T T C C A G A T G T T C A C; R: T C A G C C A T A G A A T C A C A C T T T C A; GNRHR (gonadotropin releasing hormone receptor) (116 bp), F: C A G A G C C C T T T G C C C A T A A T A; R: T G G T T A C T G A C T C C T C C A A A T G; GNRHR (103 bp), F: T G G C A C A A C T A A C A C C T A C T G; R: G A G G T A G A C A C A A G C C T G A A T T A. qPCR was performed using the Article Title: Human serum albumin and biological fluids reduce cefiderocol activity in carbapenem-resistant Gram-negative bacteria. Article Snippet: RNA extraction was performed from three independent biological replicates using the Direct-zol RNA Kit (ZymoResearch), according to the manufacturer’s instructions. .. DNase-treated RNA was used for complementary DNA (cDNA) synthesis with the iScriptTM Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). cDNA was adjusted to 50 ng/μL and used as template for qRT-PCR with qPCRBIO SyGreen Blue Mix Lo-ROX (PCR Biosystems, Wayne, PA, USA) on a Article Title: Prognostic significance and immune landscape of anoikis-related genes in hepatocellular carcinoma: a multi-omics analysis of subtypes and cellular communication. Article Snippet: RNA concentration and purity were assessed using a NanoDropTM 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA was synthesized from 100 ng of RNA using the qScriptTM cDNA SuperMix kit (Quantabio, USA). qRTPCR was performed with iTaqTM Universal SYBR® Green Supermix on a Article Title: Co-treatments of vitamin D and mesenchymal stem cells effectively alleviate the diabetic kidney disease through attenuating the SIRT1-mediated pathways. Article Snippet: Total RNA was extracted from the renal tissues and MPC5 related cells using the Trizol reagent (TaKaRa, Japan). .. Their cDNA samples were then reversely transcribed by the Transcript® One-Step gDNA Removal and cDNA Synthesis SuperMix (no. AT341-02, TransGen Biotech, China), and performed by the qRT-PCR method using the Top Green qPCR SuperMix (AQ131-01, TransGen Biotech, China) under the Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals Article Snippet: For qPCR, total RNA was extracted from 40 pooled embryos per condition using a RNeasy Kit (Qiagen). .. The purified RNAs were reverse transcribed with the iScriptTM cDNA synthesis kit (Bio-Rad) using 1 μg of total RNA per reaction. qPCR was performed with iQTMSYBR Green Supermix (Bio-Rad) and a Article Title: PEGylated liposomal fluopsin C triggers cuproptosis and ferroptosis pathways and suppresses 3D tumor spheroid growth in NCI-H460 cells. Article Snippet: Reactions contained 5 μL PowerUPTM SYBR Green Master Mix (Thermo Fisher Scientific, Cat. 01197264), primers at a final concentration of 0.45 μM each (corresponding to 0.5 μL of a 10 μM working solution), and 5 μL of diluted cDNA (1:10). .. Amplification was performed for 45 cycles on a SYBR Green Assay:Article Title: Targeted destruction of follicle stimulating hormone receptor-positive cancer cells in vitro and in vivo by a lytic peptide Phor21-FSHβ conjugate. Article Snippet: One microgram of RNA was treated with DNase I (Sigma‒Aldrich) and reverse-transcribed using the SensiFAST cDNA Synthesis Kit (Bioline, London, UK) at 48 °C for 60 min (for highly structured RNA). .. Real-time quantitative polymerase chain reaction (qPCR) was performed using the DyNAmo HS SYBR Green qPCR Kit (Thermo Fisher Scientific) with the following primers: PPIA (144 bp), F: G C C A A G A C T G A G T G G T T G G A T G; R: G A G T T G T C C A C A G T C A G C A A T G G; FSHR exon 1–2 (145 bp); F: T G G G C T C A G G A T G T C A T C A T C G G A; R: T G G A T G A C T C G A A G C T T G G T G A G G; FSHR exon 5–7 (136 bp); F: A A G C A C C T T C C A G A T G T T C A C; R: T C A G C C A T A G A A T C A C A C T T T C A; GNRHR (gonadotropin releasing hormone receptor) (116 bp), F: C A G A G C C C T T T G C C C A T A A T A; R: T G G T T A C T G A C T C C T C C A A A T G; GNRHR (103 bp), F: T G G C A C A A C T A A C A C C T A C T G; R: G A G G T A G A C A C A A G C C T G A A T T A. qPCR was performed using the Article Title: Prognostic significance and immune landscape of anoikis-related genes in hepatocellular carcinoma: a multi-omics analysis of subtypes and cellular communication. Article Snippet: RNA concentration and purity were assessed using a NanoDropTM 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA was synthesized from 100 ng of RNA using the qScriptTM cDNA SuperMix kit (Quantabio, USA). qRTPCR was performed with iTaqTM Universal SYBR® Green Supermix on a Article Title: PEGylated liposomal fluopsin C triggers cuproptosis and ferroptosis pathways and suppresses 3D tumor spheroid growth in NCI-H460 cells. Article Snippet: Reactions contained 5 μL PowerUPTM SYBR Green Master Mix (Thermo Fisher Scientific, Cat. 01197264), primers at a final concentration of 0.45 μM each (corresponding to 0.5 μL of a 10 μM working solution), and 5 μL of diluted cDNA (1:10). .. Amplification was performed for 45 cycles on a Quantitative RT-PCR:Article Title: Human serum albumin and biological fluids reduce cefiderocol activity in carbapenem-resistant Gram-negative bacteria. Article Snippet: RNA extraction was performed from three independent biological replicates using the Direct-zol RNA Kit (ZymoResearch), according to the manufacturer’s instructions. .. DNase-treated RNA was used for complementary DNA (cDNA) synthesis with the iScriptTM Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). cDNA was adjusted to 50 ng/μL and used as template for qRT-PCR with qPCRBIO SyGreen Blue Mix Lo-ROX (PCR Biosystems, Wayne, PA, USA) on a Article Title: Co-treatments of vitamin D and mesenchymal stem cells effectively alleviate the diabetic kidney disease through attenuating the SIRT1-mediated pathways. Article Snippet: Total RNA was extracted from the renal tissues and MPC5 related cells using the Trizol reagent (TaKaRa, Japan). .. Their cDNA samples were then reversely transcribed by the Transcript® One-Step gDNA Removal and cDNA Synthesis SuperMix (no. AT341-02, TransGen Biotech, China), and performed by the qRT-PCR method using the Top Green qPCR SuperMix (AQ131-01, TransGen Biotech, China) under the Synthesized:Article Title: Prognostic significance and immune landscape of anoikis-related genes in hepatocellular carcinoma: a multi-omics analysis of subtypes and cellular communication. Article Snippet: RNA concentration and purity were assessed using a NanoDropTM 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA was synthesized from 100 ng of RNA using the qScriptTM cDNA SuperMix kit (Quantabio, USA). qRTPCR was performed with iTaqTM Universal SYBR® Green Supermix on a Amplification:Article Title: PEGylated liposomal fluopsin C triggers cuproptosis and ferroptosis pathways and suppresses 3D tumor spheroid growth in NCI-H460 cells. Article Snippet: Reactions contained 5 μL PowerUPTM SYBR Green Master Mix (Thermo Fisher Scientific, Cat. 01197264), primers at a final concentration of 0.45 μM each (corresponding to 0.5 μL of a 10 μM working solution), and 5 μL of diluted cDNA (1:10). .. Amplification was performed for 45 cycles on a |